International Center for Stem Cells, Cancer and Biotechnology - icsccb

Sustained NF-κB activation allows mutant alveolar stem cells to co-opt a regeneration program for tumor initiation

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1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Delhi. Next: 24-30 March 2025, click on https://icsccb.org/workshops/cellcultureworkshop/ to apply.

Following techniques will be done hands-on by each candidate using Human Embryonic Kidney-293T and Jurkat cell lines:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

6-Days Hands-on Workshop on Molecular Biotechnology, PCR and SDS-PAGE, Delhi. Next: 5-10 May 2025. Click on https://icsccb.org/workshops/biotech-workshop/ to apply.

Following techniques will be done real hands-on by each candidate:
Recombinant DNA Technology Lab:
1. Plasmid DNA isolation
2. Plasmid DNA agarose gel electrophoresis
3. DNA extraction from agarose gel
4. Restriction digestion of DNA
5. Ligation of DNA
6. Agarose gel electrophoresis of digested and ligated DNA
7. Preparation of competent cells
8. Transformation of plasmid DNA into competent cells
9. Plating of transformed cells on LB Agar plates
10. Polymerase Chain Reaction (PCR)
11. Agarose gel electrophoresis of PCR product
12. SDS-PAGE electrophoresis of proteins
13. Coomassie staining of protein gel

Bioinformatics tools for the above techniques:
1. DNA databases
2. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
3. Designing of PCR primers,
4. Restriction analysis, restriction maps, plasmid maps,
5. Searching for homologous and paralogous sequences,
6. Promoter analysis,
7. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. It is a real hands-on training on the topics mentioned. Major focus is on the lab practicals. It will be supported by lectures in such a manner that it would help a biologist to understand the bioinformatics tools helpful in the lab and a bioinformatics person to understand the practical biotech lab techniques.

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Delhi. Next: 24-30 March 2025, click on https://icsccb.org/workshops/cellcultureworkshop/ to apply.

Following techniques will be done hands-on by each candidate using Human Embryonic Kidney-293T and Jurkat cell lines:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Very informative!

Transcription regulation by biomolecular condensates Nature Reviews Molecular Cell Biology

Hands-on Workshop on Molecular Biotechnology, PCR and SDS-PAGE, Delhi. Next: 5-10 May 2025. Click on https://icsccb.org/workshops/biotech-workshop/ to apply.

Following techniques will be done real hands-on by each candidate:
Recombinant DNA Technology Lab:
1. Plasmid DNA isolation
2. Plasmid DNA agarose gel electrophoresis
3. DNA extraction from agarose gel
4. Restriction digestion of DNA
5. Ligation of DNA
6. Agarose gel electrophoresis of digested and ligated DNA
7. Preparation of competent cells
8. Transformation of plasmid DNA into competent cells
9. Plating of transformed cells on LB Agar plates
10. Polymerase Chain Reaction (PCR)
11. Agarose gel electrophoresis of PCR product
12. SDS-PAGE electrophoresis of proteins
13. Coomassie staining of protein gel

Bioinformatics tools for the above techniques:
1. DNA databases
2. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
3. Designing of PCR primers,
4. Restriction analysis, restriction maps, plasmid maps,
5. Searching for homologous and paralogous sequences,
6. Promoter analysis,
7. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. It is a real hands-on training on the topics mentioned. Major focus is on the lab practicals. It will be supported by lectures in such a manner that it would help a biologist to understand the bioinformatics tools helpful in the lab and a bioinformatics person to understand the practical biotech lab techniques.

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Delhi, 24-30 March, 12-18 May, 10-16 Jun, 8-14 Jul 2025, click on https://icsccb.org/workshops/cellcultureworkshop/ to apply.

Following techniques will be done hands-on by each candidate using Human Embryonic Kidney-293T and Jurkat cell lines:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Cell Culture Workshop | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Cell Culture Workshop 1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, New Delhi, India Venue: ICSCCB, First Floor, Khasra 132, Plot 4, IGNOU Main Road, Near Saint Robin Public School, Neb Sarai, Saket, New Delhi – 110068, India (Nearest Metro Station is Saket on the Y...

1-Month Summer Training on Biotechnology, Animal Cell Culture and Cancer Biology, Delhi, from 1-30 May, 1-30 June, 1-30 July 2025, click on https://icsccb.org/summer-training to apply.

Following techniques (hands-on by each candidate) to be learnt during the program using Human Embryonic Kidney-293T and Jurkat cell lines:

Molecular Biology and Cell Culture Lab:
1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell line
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell line
5. Genomic DNA isolation from cancer cell line,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell line
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell line
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of SDS-PAGE gel
16. Induction of proliferation and differentiation in human cancer cell line
17. Cytospin slide preparation of hematopoietic cells
18. May-Gruenwald Giemsa Staining of cytospin slides of hematopoietic cells and visualizing morphology of differentiating cells
19. Plasmid DNA isolation
20. Plasmid DNA gel electrophoresis
21. Plasmid DNA gel extraction
22. Restriction digestion
23. Ligation
24. Agarose gel electrophoresis of restriction digested and ligated DNA
25. Preparation of competent cells
26. Transformation and plating on LBA plates

Bioinformatics tools for the above techniques:
27. DNA databases
28. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
29. Designing of PCR primers,
30. Restriction analysis, restriction maps, plasmid maps,
31. Searching for homologous and paralogous sequences,
32. Promoter analysis, Transcription factor databases,
33. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This program is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Summer/Winter Training | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Summer/Winter Training 1-Month Summer/Winter Training on “Biotechnology, Animal Cell Culture and Cancer Biology”, at the ICSCCB, New Delhi. (1 Month, Full-time, Regular Program) You can check lab photos on our posts at: https://www.linkedin.com/company/icsccb and https://www.linkedin.com/in/dr-s...

Hands-on Workshop on Molecular Biotechnology, PCR and SDS-PAGE, Delhi, 5-10 May, 2-7 June, 1-6 July 2025, click on https://icsccb.org/workshops/biotech-workshop/ to apply.

Following techniques will be done real hands-on by each candidate:
Recombinant DNA Technology Lab:
1. Polymerase Chain Reaction (PCR)
2. Agarose gel electrophoresis of PCR product
3. SDS-PAGE electrophoresis of proteins
4. Coomassie staining of protein gel
5. Restriction digestion of DNA
6. Ligation of DNA
7. Agarose gel electrophoresis of digested and ligated DNA
8. Preparation of competent cells
9. Transformation of plasmid DNA into competent cells
10. Plating of transformed cells on LB Agar plates
11. Plasmid DNA isolation
12. Plasmid DNA agarose gel electrophoresis
13. DNA extraction from agarose gel

Bioinformatics tools for the above techniques:
1. DNA databases
2. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
3. Designing of PCR primers,
4. Restriction analysis, restriction maps, plasmid maps,
5. Searching for homologous and paralogous sequences,
6. Promoter analysis,
7. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. It is a real hands-on training on the topics mentioned. Major focus is on the lab practicals. It will be supported by lectures in such a manner that it would help a biologist to understand the bioinformatics tools helpful in the lab and a bioinformatics person to understand the practical biotech lab techniques.

Biotech Workshop | International Center for Stem Cells, Cancer and Biotechnology (ICSCCB), New Delhi, India Biotech Workshop 6-Days Hands-on Workshop on Molecular Biotechnology, PCR and SDS-PAGE, New Delhi, India Venue: ICSCCB, First Floor, Khasra 132, Plot 4, IGNOU Main Road, Near Saint Robin Public School, Neb Sarai, Saket, New Delhi – 110068, India (Nearest Metro Station is Saket on the Yellow Line) ...

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay. Delhi, 12-18 May, 10-16 Jun, 8-14 Jul 2025, click on https://icsccb.org/workshops/cellcultureworkshop/ to apply.

Following techniques will be done hands-on by each candidate using Human Embryonic Kidney-293T and Jurkat cell lines:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Telomere function and regulation from mouse models to human ageing and disease Nature Reviews Molecular Cell Biology